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Yeah, he was a biologist at WHOI in Massacussetts and wanted to find the GFP gene in a jellyfish.
Do you know who is Douglas Prasher?
Exactly,to find the gene he studied the amino acid sequence of part of the GFP protein.By comparing this sequence to a genetic code table, he was able to predict aprobable mRNA base.
Do you know if he made it?
Actually, yes, after many trials he found one that bound perfectly and then he set out to find the actual gene. He found a fragments bound tightly to the mRNA that contained exactly the gene for GFP. Do you know for what is GFP?
Yeah, to label proteins in living cells and I know the method he used is called Southern blotting.
In these days is easier to scientists to search for genes in computers, the scientists make copies of it by a technique called polymerase chain reaction
I know that the first step is to heat a piece of DNA which will be separated in two strands
Yes, then primers bind to the single strands and the DNA polymerase starts coying te region between the primers
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