Gram Positive

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  • 1) Heat the slide so the bacterial cells are fixed to the slide
  • 2) Flood the slide with a crystal violet dye, which penetrate the thick peptidoglycan layer of the cell and membrane.
  • 3) Rinse with water
  • 4) Flood the slide with iodine, which penetrates the cell wall and bind with the crystal violet.
  • 5) Rinse with water again, which will stain the cell purple since the crystal violet-iodine complex is still stuck inside the cell.
  • Gram +
  • Gram -
  • 6) Decolorize the slide with alcohol
  • 7) Rinse with water
  • Gram +
  • 8) Flood the slide with Safranin dye.
  • Gram -
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