Gram Staining Storyboard-Harjap Sandhu

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  • Gram Staining Step 1: Heating the Slide
  • Gram Staining Step 2: The Dye
  • Gram Staining Step 3: Rinse
  • Heat fix the slide to ensure that the bacteria cells are fixed to the slide.
  • Gram Staining Step 4: The Iodine
  • Flood the slide with crystal violet dye- The dye ions penetrate the cell wall and membrane.
  • Gram Staining Step 5: Rinse Again
  • Rinse the slide with water.
  • Gram Staining Step 6: Decolorizing with Alcohol
  • Flood the slide with Iodine- the iodine ions penetrate the cell wall and join in with the crystal violet. This reacts and creates a complimentary large molecule that is insoluable in water
  • Rinse the slide with water.
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