Matsing and Pagong

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  • Let's move on to step 2, we join the gene unto a cloning vector.
  • Eh? What is a vector?
  • Good question, a vector is any DNA molecule which is capable of multiplying inside the host to which our gene of interest is integrated for cloning
  • Next is, introduce the vector into a suitable organism, either by Physical Gene Transfer Method, Chemical Gene Transfer Method, Transformation or Transduction
  • Then the 4th step, with the gene of interest, we would select transformed cells.
  • What's next?
  • Wow, that's very informative. What's the last step?
  • The last but not the last step, the expression or multiplication of the gene of interest.
  • That is when there would be multiple copies of the gene or to produce the protein coded by the desired gene.
  • No problem, Pagong. I had a great time. Til next time then!
  • Woah. That's a lot to remember! Thank you for the information, Matsing!
  • The pleasure is all mine! Bye! The next time we see each other, you'l be the one to teach me!
  • It was really a pleasure meeting you.
  • Alright Mara, we are finally done!!
  • Finally! I'm so tired and hungry! Come on let's go to the canteen, I'll treat you something.
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