Are You The Father?

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  • Welcome everybody to another episode of “Are You The Father!!!” Today we will be meeting a lovely lady named Barbara. 
  • Are You The Father?
  • Here’s her and Little Timmy. Little Timmy has grown up thinking John is his father but as you can see, they don’t look that alike. Let’s bring in John; Jake, a high school fling, and Jack, a random guy who walked into our studio an hour ago. 
  • Ever since Little Timmy’s birth I knew something was up, just look at our eyes! Even if it turns out that Timmy isn’t my son I will still love him regardless, I just want answers! 
  • Is this the job opening for the boom operator?
  • Barbara and I used to tell each other everything; I’d know if Little Timmy was my child. My whole life would change if the tests came back saying that I’m the father, but I’ll try my best to make up for lost time.
  • This comic portrays the concept of restriction fragment polymorphism (RFLP) with DNA fingerprinting. RFLP analysis can be done where DNA is extracted from samples, target DNA is amplified, PCR is cut with a restriction enzyme, and gel electrophoresis is performed.
  • Let’s open the floor to questions from the audience
  • I agree with John, we need answers. We all gave in our blood samples two weeks ago, what’s taking the DNA tests so long.
  • It will be expected that half of the child's DNA bands will be the same as the mother's bands and the other half of the child's bands will be the same as the true biological father's. Thirteen loci have to match as proof in court.
  • Funny story, seven years ago I was absent the whole week that we covered DNA fingerprinting and I think the rest of the audience and I would love to know how restriction fragment length polymorphism works?
  • I was thinking these questions were going to be more about-
  • Three alleged fathers involved in the paternity case.
  • Why of course, our main priority is always the audience and we have actually filmed a little video on how the whole process works, so sit back and relax as we learn who the father truly is!
  • While some DNA tests can provide results in 1-2 days, others can take ~3-12 weeks.
  • DNA fingerprinting is a method used to identify an individual based on their unique patterns in DNA. RFLP is a variation in the length of restriction fragments produced by a given restriction enzyme in a sample of DNA. 
  • We will explain how just one band disproves paternity and also explain how the test can never absolutely prove paternity. For statistically significant evidence of a match in a court of law, 13 different regions of genome must be analyzed.
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