OM45- GFP

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  • Introduction: Membrane protein insertion OM 45 Fusion proteins GFP, localisaiton, membrane insertion
  • Methods Cloning of GFP, Amplification of OM45 Expression of fusion proteins Purification Ni column, FPLC Characterization by PAGE, ESI MS, Fluorescence, Absorbance Molecular Dynamic simulations Vesicle preparation, Localization
  • Results, Cloning and expression Characterization using PAGE, Native PAGE,  ESI MS Fluorescence of GFP, MD studies
  • Pending: Vesicle studies Localization, in vivo localization 
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