DNA Replication

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  • DNA Replication
  • The strands are called three prime and five prime depending on the direction their corresponding nucleotides were combined.
  • The enzyme helicase unzips the two helixes of the DNA by unraveling it.
  • 3'
  • 5'
  • The leading strand (3') goes on to be transformed into DNA polymerase (III). The polymerase is used as a template for the formation of daughter DNA.
  • The lagging strand (opposite of 3' to 5' orientation) is organized into Okazaki fragments. From there they are sent to a DNA polymerase (in 5' to 3' orientation). Then the fragments are synthesized in reverse.
  • Okazaki
  • 3'
  • 5'
  • DNA polymerase I replaces the last nucleotide of RNA with a DNA nucleotide and leaves the backbone (sugar-phosphate) with a 3' end.
  • DNA polymerase III completes the synthesis of fourth Okazaki fragment. On the third fragment, as the polymerase comes to the RNA primer, it will dis-attach, then return to the replication fork and add nucleotides the 3' end of the fifth RNA fragment primer.
  • pol I
  • Enzyme ligase creates a bond between the second fragment's 3' to the first fragment's 5'.
  • ligase
  • 5'
  • 3'
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