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  • Take the 3 microcentrifuge tubes and add 100μL of water. Also add 50μL of iodine. Cap. Shake to mix. 
  • Obtain your banana sample. The key is to not use too much so you can see individual cells. Use the yellow inoculating loop and scrape the banana gently so that the loop is filled
  • banana paste
  • inoculating loop
  • Use a pipette to transfer 1 drop of solution to your slide. 
  • then Place a coverslip on top
  • Place the slide on the microscope, start with with 4x (red) to locate cells and move to 10x (yellow) objective in position and find a field of view containing the cells. 
  • Place the slide on the microscope, start with with 4x (red) to locate cells and move to 10x (yellow) objective in position and find a field of view containing the cells. 
  • You will see cells in your field of view. Use 5 cells in your field of view and count the plastids inside. Make 4 counts and average them. Log into your data chart
  • Repeat 1-5 again for the ripe and overripe bananas. 
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