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Gram staining 2
Updated: 1/27/2020
Gram staining 2
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Storyboard Text

  • Using a circular motion, mix the bacterial culture into the drop of water on the slide. You want to make a uniform smear in the center portion of the slide.
  • Place the used inoculation loop in the beaker containing the disinfectant. Be sure the portion of the loop that contacted the bacteria is completely submerged in the disinfectant.
  • Allow the water to completely evaporate from the slide. It is important that the slide air dries. After the water evaporates, you may not see anything on the slide, or you may see a slight haze.
  • Light the alcohol lamp.Use a test tube clamp or clothes pin to hold the slide, as demonstrated by your teacher.
  • Make sure your hair and clothing are safely away from the flame.
  • Carefully pass the center portion of the slide through the flame three times, as demonstrated by your teacher.
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