She has spoke what she should not, I am sure of that.
What's done cannot be undone,—to bed,to bed, to bed!
In this scene, Lady Macbeth reveals the murder of King Duncan and her inner guilt as she is sleep walking. This is an important moment in the play because it shows that Lady Macbeth's level of evil has lessened compared to her evil in Act 1. In the beginning, Lady Macbeth is portrayed as masculine and confident when she is preparing the murder of King Duncan. However, Lady Macbeth's change shows that she is not as masculine as first portrayed, she is more feminine-like now due to the immense guilt she bears from her actions.
As Malcolm and Macduff plan to invade Macbeth's kingdom, they cut off branches from the Birnam Woods in order to conceal their army's numbers. This is a moment of agnorsis because when Macbeth hears the prophecy about Birnam woods marching to his Kingdom, he is overconfident that such a thing cannot occur. But now Birnam woods is indeed marching Macbeth's kingdom and his fears have increased.
In the final scene of play, Maduff and Macbeth duel to the death. This is another moment of agnorsis because Macbeth learns that Macduff was born from a C-section, hence, not born from a woman. Macbeth's fear increases once again, he was overlyconfident that all people are born of a woman, but he did not take a C-section into account because he is blinded by his ambition and hubris. The play then ends with Macduff beheading Macbeth and Malcolm being crowned King of Scotland.