Wearing goggles, connect the gas pressure tube to the valve and set up Lab Quest. Then get four labeled test tubes and placed 3 ml of H202 and 3 ml of water in them.
Add yeast to the test tubes and put a stopper on them so you can swirl the test tube and mix the substances. Connect the stopper to the tubing.
Start collecting data (do not go over 130 kpa) and determine the rate of the enzyme activity (pressure v. time). Collect data for other 3 test tubes with 2, 3, and 4 yeast spheres. Graph data
Get 4 clean test tubes and label them. Add H202 and water. Measure enzyme activity by preparing a water bath. For 0-5 use ice and water in beaker; for 20-25 use warm water; for 50-55 use hot water (check temp with a thermometer). Add two yeast spheres and measure enzyme activity.
Get 3 clean test tubes and label them (pH 4, 7, 10). Add H202 and water. Add 2 yeast spheres to each tube and measure the enzyme activity.
Make sure to graph data of temperature change and pH change affects on the enzyme activity, and record the data in the table.