Lab U3 Enzymes

Lab U3 Enzymes
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  • Wearing goggles, connect the gas pressure tube to the valve and set up Lab Quest. Then get four labeled test tubes and placed 3 ml of H202 and 3 ml of water in them.
  • Add yeast to the test tubes and put a stopper on them so you can swirl the test tube and mix the substances. Connect the stopper to the tubing.
  • Start collecting data (do not go over 130 kpa) and determine the rate of the enzyme activity (pressure v. time). Collect data for other 3 test tubes with 2, 3, and 4 yeast spheres. Graph data
  • Get 4 clean test tubes and label them. Add H202 and water. Measure enzyme activity by preparing a water bath. For 0-5 use ice and water in beaker; for 20-25 use warm water; for 50-55 use hot water (check temp with a thermometer). Add two yeast spheres and measure enzyme activity.
  • Get 3 clean test tubes and label them (pH 4, 7, 10). Add H202 and water. Add 2 yeast spheres to each tube and measure the enzyme activity.
  • Make sure to graph data of temperature change and pH change affects on the enzyme activity, and record the data in the table.
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