Updated: 7/22/2020
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  • Hey Matthew! Are you ready for tomorrow's test on glycolysis?
  • I think so! Want to review it together on the way home?
  • Awesome!! I'll start. In essence, glycolysis is the ten-step conversion of glucose to 2 pyruvate molecules.
  • Followed by step 2; an isomerization reaction. Here, glucose-6-phosphate is rearranged into its isomer fructose-6-phosphate.
  • In step 1, a phosphorylation reaction occurs. This is where the glucose turns into glucose-6-phosphate.
  • Right! Then in step 3, fructose-1,6-biphosphate is created through phosphorylation when a phosphate group from ATP attaches to fructose-6-phosphate.
  • Next, step 4, is a lysis reaction occurs which splits the fructose-1,6-biphosphate into glyceraldehyde-3-phosphate & dihydroxyacetone phosphate
  • In step 5, an isomerization reaction occurs. This converts the DHAP G3P, resulting in 2 molecules from each glucose.
  • This energy may be received by an inorganic phosphate group. NAD+, will collect 2 electrons & 1 proton to create NADH. Cytosol will accept the other proton.
  • Then in step 6, 2 electrons & protons are removed from G3P, which causes energy to be released.
  • In the next step, a substrate level phosphorylation reaction occurs. One of the phosphate groups from 1,3-biphosphoglycerate is transferred to ADP to create ATP
  • In step 8, a mutase reaction produces 2-phoshoglycerate.
  • The rearrangement of 3-phosphoglycerate shifts the phosphate group from the 3-carbon to the 2-carbon.
  • In step 9, electrons from 2-phosphoglycerate are moved to different location in the molecule. Water is lost and phosphoenolpyruvate will retain majority of the lost energy.
  • Yay! We got it! And the final products are 4 ATP (net2), 2 NADH, and 2 pyruvates!
  • Then in the last step, the phosphate group is removed from the phosphoenolpyruvate and transferred to ADP. This will form ATP & pyruvate!
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