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Identify a section of DNA that contains the gene for making the desired protein from the chromosome. Remove the required gene using an enzyme
Collect bacterial cell and remove plasmid. Open the plasmid using the same enzyme as was used for the gene
Insert the gene into the open plasmid and close using a different enzyme.
Insert the plasmid back into the same host bacterial cell to produce a genetically modified (GM) organism.
Grow the bacteria to have lots of bacterial cells containing the gene to make the new protein.
Extract and purify the protein for use.
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