John White and his colonists sailed to the island to investigate. The colonists including John White's pregnant daughter planned to explore before and while John White leaves for supplies.
The colonists waited too long for White and returned to England with Sir Frances Drake. When John White returned, the island was completely deserted.
I believe that the colonists left to Croatoan , but they were lost at sea on their way there. I believe that because they carved out the island on the tree and probably used it as a clue to tell the others. But there were no bodies found which means they could have got lost at sea due to natural disaster since there were a lot of hurricanes.