The story begins in 1989, centred on troublesome student Jonathan Kujo with Holly, his mother. Joseph, Holly's father, moves to Japan after hearing Jonathan has been arrested for hospitalizing three armed men and a trained boxer.
Jonathan refuses to leave his cell, claiming that their injuries were caused by an evil spirit possessing him. Joseph arrives with Mohammed Avdul.
The first assassin is brain-washed student Kakyoin, who uses the amorphous Snake Stand. Jonathan defeats him and rescues from Dio’s vampiric influence.
Another brain-washed assassin, the Frenchman Jean Pierre Polnareff, user of the Knight Stand, is saved and recruited into the group.
The surviving heroes confront Dio, but Dio soon escapes after the room fills up with sunlight, his biggest weakness.
Jonathan and Joseph bid farewell to Polnareff before taking the next flight to Japan, as a recovering Holly awakens back at home.