Procedure of paper chromatography
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waiting 20 minutes
Paper chromatography 1.5 cm from the bottom Paper chromatography 1.5 cm from the bottom
sample 3 sample 3
placing paper will dotted sample and waiting 20 minutes
removing paper from the solvents and allow to full dry
spraying 0.5% ninhydrin on the paper
circle the colored mark
marking the center of the circle and finding the migrated distance
Using the dashed line to locate the solvent front line and calculate Rf value for amino acidsds