Procedure of paper chromatography

Updated: 10/6/2021
Procedure of paper chromatography

Storyboard Text

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  • Paper chromatography
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  • waiting 20 minutes
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  • Paper chromatography 1.5 cm from the bottom Paper chromatography 1.5 cm from the bottom
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  • sprayer
  • 0.5% ninhydrin
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  • placing paper will dotted sample and waiting 20 minutes
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  • removing paper from the solvents and allow to full dry
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  • spraying 0.5% ninhydrin on the paper
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  • circle the colored mark
  • marking the center of the circle and finding the migrated distance
  • Using the dashed line to locate the solvent front line and calculate Rf value for amino acidsds