How can you get uncontaminated culture

How can you get uncontaminated culture
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  • Today we are going to do a required practical: How can you get uncontaminated culture
  • Firstly, get a petri dish and put it near a flame so no bacteria from the surroundings get in the dish therefore ensuring it does not get contaminated
  • Secondly, get an inoculating loop and sterilise it by hovering it over a flame
  • Thirdly, get your chosen bacteria and carefully spread it over the agar jelly making sure that you don't create any dents in it
  • Then, close the petri dish and seal it so that the culture does not get contaminated
  • Finally, place the petri dish upside down in a water bath at 25 degrees to obtain uncontaminated culture
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