Koch would go on to further study Anthrax, would refine and create new bacteriological methods, would discover the tubercle bacillus
Koch set himself to prove scientifically that Anthrax bacillus is, in fact, the cause of the disease. He inoculated mice, with anthrax bacilli taken from the spleens of farm animals that had died of anthrax, and found that these mice were all killed by the bacilli, whereas mice inoculated at the same time with blood from the spleens of healthy animals did not suffer from the disease.
Koch grew the bacilli for several generations and showed that, although they had had no contact with any kind of animal, they could still cause anthrax. The results of this painstaking work were demonstrated by Koch to Ferdinand Cohn, Professor of Botany who published Koch's work in the botanical journal of which he was the editor. Koch immediately became famous.