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Rolling Circle Mode of Replication
Storyboard-Text
- David H Dressler , Come on lets duplicate the circular DNA
- Helicase
- DNA Polymerase
- RNA Polymerase
- Primase
- Okayyyyy
- DNA Gyrase
- SSBP
- DNA Ligase
- The DNA is Circular,who is going to cut to start the work
- I am Helicase, I have Rep A Scissors to cut the outer circle at Ori
- Good Job!! Helicase, Now if the 3'OH end acts as primer, Who will continue next?
- I DNA polymerase III can help to synthesize the new strand using the inner circle as template
- 3'OH
- 5'P + Rep A
- SSBP
- Hey DNA pol, don't worry I'll make sure that the unwinded DNA does not rewind and even prevent from looping.
- Hey Helicase and SSBP its urgent .. can you help please?
- Yeah sure, I will unwind the circular DNA
- DNA Ligase
- Good work all! Now, who is willing to seal the nicks?
- I will seal the double stranded DNA using the Phosphodiester glue
- GLUE
- I will seal the Single stranded DNA using Rep A
- TEMPLATE STRAND
- NEWLY SYNTHESIZED STRAND
- This is something similar to Robin Holliday recombination model in fungi. Maybe what I found is right
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