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- Oh yeah! It took us a while to find the magic taco, but we did it. Yes, we finally found the magic taco. Spectroscopy allows us to analyze it at last.
- As soon as we get back to the lab, I'll explain it to you
- Spectro.... what? What is that?
- Spectroscopy is the study of the interaction between matter and electromagnetic radiation. This interaction can take many forms, including absorption, emission, scattering, and reflection of electromagnetic radiation. One of the key tools used in spectroscopy is the absorption spectrum, which is a graph or plot showing the amount of light absorbed by a sample as a function of the frequency or wavelength of the light. The absorption spectrum is used to identify the molecules or atoms present in a sample, as well as to determine their concentration and other physical and chemical properties. By studying the absorption spectrum of a sample, scientists can learn a great deal about the composition and properties of the sample.
- Does Beer-Lambert have anything to do with this?
- Yes it does! The Beer-Lambert law states that the absorbance of a solution is directly proportional to the concentrationof the absorbing species in the solution. In other words, the law states that the amount of light absorbed by a solution is directly proportional to the concentration of the absorbing species in the solution. This relationship isdescribed by the equation A = ε*c*l,where A is the absorbance of the solution, ε is the molar absorptivity of the absorbingspecies, c is the concentration of the absorbing species in the solution, and lis the path length of the light through the solution.
- You are right, it has many applications in medicine. It could be used for diagnosis of diseases by analysing the absorption and emission spectra of biological samples. It is also used to in monitoring of drug concentration, analysis of tissue samples and even imaging of the human body with techniques, such as MRI and CT scans.
- So spectroscopy must have a wide range of applications in medicine right?
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