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DNA#1

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DNA#1
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  • HELLO, my name is Frederick Griffith
  • There are 2 forms of this bacteriumpathogenic strainnon-pathogenic strain
  • I am to develop a vaccine for pneumonia. Which is caused by a bacteria Streptococcus pneumoniae
  • 1928
  • A live form of the pathogenic strain
  • 1
  • A non-pathogenic strain
  • 2
  • My experiment will be done with mice.
  • Heat killed pathogenic strain(bacteria)
  • Here is what I will inject into each of them
  • 3
  • Heat killed pathogenic strain
  • 4
  • +
  • Live non-pathogenic strain
  • As expected Mice #1 died and Mice #2 lived. Mice #3 which had the pathogenic strain that I killed using heat, indeed did live and suffered no effects.
  • 1
  • I was shocked at the results of Mice #4. I had injected a mix of the heat killed pathogenic bacteria with the live non-pathogenic bacteria. I had thought the mice would live because the pathogenic strain is dead BUT Mice #4 is dead.
  • x
  • 2
  • 3
  • x
  • 4
  • TRANSFORMATION
  • I propose that one of the chemicals in the heat that was used to kill the pathogenic strain altered the live non-pathogenic strain. This caused it to be deadly.
  • I called this phenomenon of passing on genetic material transformation!
  • We will continue the work of Frederick Griffith and determine the the isolating agent behind the transforming principle
  • Avery
  • MacLeod
  • Mid 1940's
  • is it DNA or protein?
  • McCarty
  • We will reproduce Griffith experiment. But, have one important difference
  • 1
  • 2
  • 3
  • 4
  • Mice #5 we will treat the bacteria with a protein destroying enzyme
  • Mice #6 the bacteria will be treated with a DNA-destroying enzyme
  • 5
  • 6
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