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llungs 2

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llungs 2
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  • Antioxidant and CAR T-cell Therapy to Repair Lung Tissue Damage due to the Use of E-cigarettes
  • Hello! Welcome to our grant proposal where we are pitching a new innovative therapy!
  • We will use a CAR T-Cell therapy to overproduce IL-22 and stimulate epithelial cell regeneration.
  • CAR T-cell
  • Overview
  • Liposomal Nanoparticles
  • We also plan to use liposomal nanoparticles to introduce antioxidants that donate their excess electrons to neutralize free radicals.
  • HA! That question stumped em!
  • CAR T-cell- IL -22- Epithelial Regeneration
  • HOW DO YOU PLAN ON TESTING THIS?
  • Overview
  • Liposomal Nanoparticles- Antioxidants-Neutralize Free radicals
  • I know the answer, do they???
  • To test our CAR T cell approach, we will study cell proliferation after exposure to IL-22. Different groups will be exposed to varying levels of IL-22 concentration in order to interpret the conclusions.
  • Proliferation StudyIncreased IL-22 LevelsImpact on Epithelial Lung Tissue
  • CAR T-cell In Vitro Experiment
  • Nanoparticle Antioxidant In Vivo Experiment
  • To test our antioxidant approach, we will expose mice to e-cigarette aerosols, specifically nicotine and then inject the mice with the nanoparticles containing antioxidants. We will then dissect, stain, and analyze mice lungs and compare between 4 test groups: 2 control and 2 experimental.
  • Expected Outcomes
  • Reduced presence of free radicalsLung epithelial tissue regeneration
  • WE ARE SO IMPRESSED! Please take the millions!!
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